LOCUS       IS26-mph(A)-IS6100_unit 5055 bp    DNA     linear       03-OCT-2020
DEFINITION  Citrobacter freundii.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Unclassified.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of IS26-mph(A)-IS6100 unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
     repeat_region   1..14
                     /gene="IRL_IS26"
     CDS             64..768
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   807..820
                     /gene="IRR_IS26"
     CDS             890..1795
                     /gene="mph(A)"
                     /codon_start=1
                     /product="Macrolide phosphotransferase"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     CDS             1792..3030
                     /gene="mrx(A)"
                     /codon_start=1
                     /product="Positive regulator"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     CDS             3030..3614
                     /gene="mphR(A)"
                     /codon_start=1
                     /product="Negative regulator"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     repeat_region   complement(4053..4066)
                     /gene="IRR_IS6100"
     CDS             complement(4107..4871)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS6100 transposase"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   complement(4919..4932)
                     /gene="IRL_IS6100"
     repeat_region   5031..5055
                     /gene="IRt"
ORIGIN      
        1 ggcactgttg caaatagtcg gtggtgataa acttatcatc cccttttgct gatggagctg
       61 cacatgaacc cattcaaagg ccggcatttt cagcgtgaca tcattctgtg ggccgtacgc
      121 tggtactgca aatacggcat cagttaccgt gagctgcagg agatgctggc tgaacgcgga
      181 gtgaatgtcg atcactccac gatttaccgc tgggttcagc gttatgcgcc tgaaatggaa
      241 aaacggctgc gctggtactg gcgtaaccct tccgatcttt gcccgtggca catggatgaa
      301 acctacgtga aggtcaatgg ccgctgggcg tatctgtacc gggccgtcga cagccggggc
      361 cgcactgtcg atttttatct ctcctcccgt cgtaacagca aagctgcata ccggtttctg
      421 ggtaaaatcc tcaacaacgt gaagaagtgg cagatcccgc gattcatcaa cacggataaa
      481 gcgcccgcct atggtcgcgc gcttgctctg ctcaaacgcg aaggccggtg cccgtctgac
      541 gttgaacacc gacagattaa gtaccggaac aacgtgattg aatgcgatca tggcaaactg
      601 aaacggataa tcggcgccac gctgggattt aaatccatga agacggctta cgccaccatc
      661 aaaggtattg aggtgatgcg tgcactacgc aaaggccagg cctcagcatt ttattatggt
      721 gatcccctgg gcgaaatgcg cctggtaagc agagtttttg aaatgtaagg cctttgaata
      781 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
      841 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      901 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      961 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
     1021 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
     1081 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
     1141 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
     1201 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
     1261 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
     1321 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
     1381 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
     1441 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
     1501 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
     1561 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
     1621 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
     1681 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
     1741 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1801 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1861 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1921 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1981 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     2041 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     2101 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     2161 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     2221 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     2281 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     2341 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     2401 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     2461 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     2521 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     2581 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     2641 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     2701 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     2761 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2821 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2881 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2941 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     3001 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     3061 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     3121 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     3181 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     3241 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     3301 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     3361 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     3421 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     3481 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     3541 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     3601 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     3661 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     3721 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3781 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3841 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3901 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3961 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     4021 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     4081 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     4141 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     4201 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     4261 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     4321 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     4381 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     4441 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     4501 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     4561 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     4621 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     4681 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     4741 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4801 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4861 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4921 tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcagatc acttctgata
     4981 ttgaactgtc aggagctggc tgcacaacag ccattacgcc caatcaactg gtgcagtcgt
     5041 cttctgaaaa tgaca
//
